Malaria Breakthrough as Lab Fungus Kills 99% of Mosquitoes

A team of scientists from the University of Maryland (UMD) and Burkina Faso have conducted the first outside-of-a-laboratory trial of a transgenic method for combating malaria. The method, which kills mosquitoes with a fungus genetically engineered to produce spider toxin, is the latest step forward in UMD work to develop powerful new bioinsecticides and biopesticides through the creation of transgenically altered fungi.

In a research paper published on May 30, 2019 in the journal Science,  the UMD and Burkina Faso scientists describe their successful trial conducted in a screen-enclosed, simulated village setting in Burkina Faso, West Africa. Using a transgenic fungus developed by a UMD team led by Distinguished University Professor of Entomology Raymond St. Leger, mosquito populations were safely reduced in the trial by more than 99 percent.

According to the World Health Organization, malaria affects hundreds of millions of people around the world, killing more than 400,000 annually. Decades of insecticide use has failed to control mosquitoes that carry the malaria parasite and has led to insecticide-resistance among many mosquito strains. For more than a decade, St. Leger and other scientists have been genetically modifying mosquitoes and other organisms that could help eradicate mosquitoes. However, until now, none of these transgenic approaches had made it beyond laboratory testing.

“No transgenic malaria control has come this far down the road toward actual field testing,” said Brian Lovett, a graduate student in UMD’s Department of Entomology who works with St. Leger and is the lead author of the new paper. “This paper marks a big step and sets a precedent for this and other transgenic methods to move forward.”

“We demonstrated that the efficacy of the transgenic fungi is so much better than the wild type that it justifies continued development,” said St. Leger, a co-author of the study.

Next, the international team of scientists hope to test their transgenic fungus in a local village or community. There are many regulatory and social benchmarks to meet before deploying this new method in an open environment such as a village, but the researchers said this study helps make the case for such trials.

The fungus used by the researchers is a naturally occurring pathogen that infects insects in the wild and kills them slowly. It has been used to control various pests for centuries. The scientists used a strain of the fungus that is specific to mosquitoes and engineered it to produce a toxin that kills mosquitoes more rapidly than they can breed. This transgenic fungus caused mosquito populations in their test site to collapse to unsustainable levels within two generations.

“You can think of the fungus as a hypodermic needle we use to deliver a potent insect-specific toxin into the mosquito,” said St. Leger.

The toxin is an insecticide called Hybrid. It is derived from the venom of the Australian Blue Mountains funnel-web spider and has been approved by the Environmental Protection Agency (EPA) for application directly on crops to control agricultural insect pests.

“Simply applying the transgenic fungus to a sheet that we hung on a wall in our study area caused the mosquito populations to crash within 45 days,” Lovett said. “And it is as effective at killing insecticide-resistant mosquitoes as non-resistant ones.”

Lovett said laboratory tests suggest that the fungus will infect the gamut of malaria-carrying mosquitoes. The abundance of species that transmit malaria has hindered efforts to control the disease, because not all species respond to the same treatment methods.

To modify the fungus Metarhizium pingshaense so that it would produce and deliver Hybrid, the UMD research team used a standard method that employs a bacterium to intentionally transfer DNA into fungi. The DNA the scientists designed and introduced into the fungi provided the blueprints for making Hybrid along with a control switch that tells the fungus when to make the toxin.

The control switch is a copy of the fungus’ own DNA code. Its normal function is to tell the fungus when to build a defensive shell around itself so that it can hide from an insect’s immune system. Building that shell is costly for the fungus, so it only makes the effort when it detects the proper surroundings—inside the bloodstream of a mosquito.

By combining the genetic code for that switch with the code for making Hybrid, the scientists were able to ensure that their modified fungus only produces the toxin inside the body of a mosquito. They tested their modified fungus on other insects in Maryland and Burkina Faso, and found that the fungus was not harmful to beneficial species such as honeybees.

“These fungi are very selective,” St. Leger said. “They know where they are from chemical signals and the shapes of features on an insect’s body. The strain we are working with likes mosquitoes. When this fungus detects that it is on a mosquito, it penetrates the mosquito’s cuticle and enters the insect. It won’t go to that trouble for other insects, so it’s quite safe for beneficial species such as honeybees.”

After demonstrating the safety of their genetically modified fungus in the lab, Lovett and St. Leger worked closely with scientific colleagues and government authorities in Burkina Faso to test it in a controlled environment that simulated nature. In a rural, malaria-endemic area of Burkina Faso, they constructed a roughly 6,550-square-foot, screened-in structure they called MosquitoSphere. Inside, multiple screened chambers contained experimental huts, plants, small mosquito-breeding pools and a food source for the mosquitoes.

In the chamber containing the sheet treated with the transgenic fungus, mosquito populations plummeted over 45 days to just 13 adult mosquitoes. That is not enough for the males to create a swarm, which is required for mosquitoes to breed. By comparison, the researchers counted 455 mosquitoes in the chamber treated with wild-type fungus and 1,396 mosquitoes in the chamber treated with plain sesame oil after 45 days. They ran this experiment multiple times with the same dramatic results.

In similar experiments in the lab, the scientists also found that females infected with transgenic fungus laid just 26 eggs, only three of which developed into adults, whereas uninfected females laid 139 eggs that resulted in 74 adults.

According to the researchers, it is critically important that new anti-malarial technologies, such as the one tested in this study, are easy for local communities to employ. Black cotton sheets and sesame oil are relatively inexpensive and readily available locally. The practice also does not require people to change their behavior, because the fungus can be applied in conjunction with pesticides that are commonly used today.

“By following EPA and World Health Organization protocols very closely, working with the central and local government to meet their criteria and working with local communities to gain acceptance, we’ve broken through a barrier,” Lovett said. “Our results will have broad implications for any project proposing to scale up new, complex and potentially controversial technologies for malaria eradication.”

Read more at the University of Maryland

The Government Technology & Services Coalition's Homeland Security Today (HSToday) is the premier news and information resource for the homeland security community, dedicated to elevating the discussions and insights that can support a safe and secure nation. A non-profit magazine and media platform, HSToday provides readers with the whole story, placing facts and comments in context to inform debate and drive realistic solutions to some of the nation’s most vexing security challenges.

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